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Image Search Results
Journal: Molecular Therapy. Nucleic Acids
Article Title: miR-140 Attenuates the Progression of Early-Stage Osteoarthritis by Retarding Chondrocyte Senescence
doi: 10.1016/j.omtn.2019.10.032
Figure Lengend Snippet: Intra-articular Injection (IAJ) of miR-140 Attenuates OA Progression in Rats (A) Experimental layout for the in vivo experiments in rats. (B) Representative in vivo fluorescence image 24 h after IAJ of Cy5-labeled miR-140 agomir into the right hind knee and normal saline (NS) injection into the left hind knee. (C) Representative fluorescence micrographs of sagittal frozen sections of the medial femoral condyles 24 h after IAJ of NS into the left hind knee (top) and Cy5-labeled miR-140 agomir into the right hind knee (bottom; 100× scale bars, 200 μm; 400× scale bars, 50 μm). (D) Number of rears, as measured by behavioral testing, before and after IAJ of miR-Scr or miR-140 agomir. (E–K) Gross observation (E), pathological lesion score (F), representative sagittal micrographs of H&E (G), and Safranin O (H) staining (scale bars, 100 μm) and modified Mankin score (I), cartilage thickness (J), and chondrocyte number (K) of the medial femoral condyles before and after IAJ of miR-Scr or miR-140 agomir. ACLT + DMM, anterior cruciate ligament transection (ACLT) and destabilization of the medial meniscus (DMM). *p < 0.05, **p < 0.01, ***p < 0.001.
Article Snippet: The miR-140 agomir and
Techniques: Injection, In Vivo, Fluorescence, Labeling, Saline, Staining, Modification
Journal: Molecular Therapy. Nucleic Acids
Article Title: miR-140 Attenuates the Progression of Early-Stage Osteoarthritis by Retarding Chondrocyte Senescence
doi: 10.1016/j.omtn.2019.10.032
Figure Lengend Snippet: Intra-articular Injection (IAJ) of miR-140 Retards Chondrocyte Senescence in Rats (A–D) Representative micrographs of immunohistochemical staining and immunopositive percentage of p16 INK4a (A), p21 (B), p53 (C), and γH2AX (D) in sagittal sections of the medial femoral condyles after IAJ of miR-Scr or miR-140 agomir (scale bars, 100 μm for p16 INK4a , p21, and p53; 50 μm for γH2AX). *p < 0.05, **p < 0.01, ***p < 0.001.
Article Snippet: The miR-140 agomir and
Techniques: Injection, Immunohistochemical staining, Staining